Rice qUVR-10, a quantitative trait locus (QTL) for ultraviolet-B (UVB) resistance on chromosome 10, was cloned by map-based strategy.The dimer causes local distortions in the helix, weakening the interaction with the paired adenine bases and kinking the backbone slightly. Notice the four‐membered cyclobutane ring formed between the two thymine bases. A TT dimer (in violet) is shown within a DNA double helix. UV-irradiated or unirradiated pZ189 was mixed with photolyase and treated as described (25). The enzyme had no endonucleolytic activity on unirradiated plasmid. Photoly-ase specifically monomerizes cyclobutane pyrimidine dimers but does not modify pyrimidine-pyrimidone(6-4) photoprod-ucts (24). dimer removal from UV-irradiated pZ189 plasmid.
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